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The use
of enzyme labeled antibodies for localization of cellular antigens
in tissue sections by using light or electron microscopy has
become a technique of choice for many investigators.
In order
to obtain optimal results with immunoenzymatic techniques, it
is essential that labeled antibodies are stable preparations,
show good tissue penetration for maximum sensitivity, and show
little or no background staining.
Protos
Immunoresearch has prepared Peroxidase conjugated Fab' fragments
of affinity purified antibodies that meet these requirements.
| Tissue Penetration |
| Fab' -peroxidase conjugates
are |
| chromatographically separated to
yield |
| conjugate with1:1 enzyme:antibody |
| ratio. The low molecular weight (~87,000)
|
| insures excellent tissue penetration. |
| |
| STABILITY |
| Fab' fragments are covalently coupled with |
| horseradish peroxidase (RZ 3.0 min.)
|
| to produce conjugates that are stable for |
| at least 1 year at +4oC. |
| |
| SPECIFIC STAINING |
| Since Fab' fragments are derived |
| from affinity purified antibodies, all |
| conjugates are antigen-directed and |
| produce specific staining. |
|
| Background Staining |
| Minimal due to: |
| 1. low molecular weight of conjugate |
| 2. absence of Fc fragment |
| 3. absence of components that can |
| nonspecifically
stain (e.g. biotin, avidin) |
| |
| ADVANTAGES OF FAB'-PEROXIDASE |
| CONJUGATES OVER THE (STREPT) |
| AVIDIN-BIOTIN SYSTEM |
| * controlled size- conjugates |
| * only one reagent needed. Shorter |
| incubation and washing times. |
| * Absence of biotinylated antibody
and |
| (strept) avidin, both can
cause |
| non-specificstaining. |
| |
| |
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Home >>
Technical Information >>
Technical Information on Enzyme Antibody Conjugates
>> Peroxidase Conjugated Fab' Fragments of Affinity
Purified Antibodies
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