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Technical Information on R-Phycoerythrin Conjugated Fab¢ Fragments Phycoerythrin and other
phycobiliproteins are well characterized when used as conjugates (usually
coupled to antibodies) in fluorescent immunoassays. They possess many
fluorescent groups per protein resulting in large extinction coefficients and
high quantum yields. Other desirable characteristics are:
PROTOS IMMUNORESEARCH provides Fab¢ fragments of secondary antibodies, coupled to R-Phycoerythrin (R-PE). Fab¢ fragments were chosen since they are well suited to be covalently coupled to R-PE, while preserving antibody activity. In addition, absence of the Fc part will avoid Fc-mediated non-specific binding to cells and tissues. On average, between two and three molecules of Fab¢ are coupled to one R-PE molecule. Due to the coupling technique used, larger complexes are avoided, resulting in very low background levels. R-PE : Mol.Weight 240,000 dalton Absorption : 488 nm ; Emission : 575 nm Conjugate : 0.2 mg/ml Buffer : PBS, pH 7.2, 1% BSA, 15 mM sodium azide (preservative) Application : Stain 1 Million cells in Flow Cytometry with 1 mg conjugate . (lesser amounts may be sufficient)
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