Technical
Information on Biotin Antibody Conjugates
The high affinity binding of avidin with
biotin provides a unique approach to antibody localization. PROTOS
IMMUNORESEARCH supplies affinity purified antibodies and F(ab')2
or Fab' antibody fragments conjugated to biotin. These reagents
can be localized by utilizing (strept)avidin conjugates that specifically
bind biotin. Experience has shown that biotin conjugates are sensitive
and specific tools in immunohistology, immunocytology, ELISA and immunoblotting.
The working dilution of biotin conjugated antibodies in
ELISA is 1:10,000 - 1:20,000.
Introduction of Biotin groups in Monoclonal Antibodies
Biotin conjugated goat Fab'anti-mouse IgG(H+L) [Cat.No: 648] can
be used to introduce biotin groups into mouse monoclonals:
- Dilute the monoclonal antibody to 200 ng/ml in PBS. (1:5000 when
starting from 1 mg/ml).
- Add Cat.648 to a final conc. of 1:1000. Mix well.
- Incubate 30 minutes at room temperature.
- Bind excess Cat.648 by adding 1:100 final normal mouse serum. Mix
well.
- Incubate 30 minutes at room temperature.
Since excess Cat.648 has been removed, the now biotin conjugated
monoclonalantibody can be used on a variey of proteins (eg Western
Blots) and tissues, including mouse.
In a similar fashion, biotin conjugated goat Fab' anti-rat IgG(H+L)
[Cat.No: 687] can be used to introduce biotin groups into rat monoclonals.
Excess Cat.687 will be removed with 1:100 final normal rat serum.
Protos Immunoresearch
|
875A Cowan Road, Burlingame, CA 94010
|
Tel: 650 652-3450
Fax: 650 652-3451
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