Information on Fluorochrome Antibody Conjugates
Affinity purified antibodies labelled with fluorochromes
are time-proven markers of tissue and plasma membrane antigens. For
purposes of immunocytological investigations, PROTOS IMMUNORESEARCH
provides antibody conjugates of fluorescein (FITC), rhodamine (TMRITC)
and Texas Red. These can be used as individual reagents or in double
antibody experiments in fluorescence microscopy, flow cytometry and
fluorometric immunoassays. Combinations of FITC and Texas Red conjugates
are well suited for flow cytometry, because their emission spectra
are widely separated (i.e., 515 nm vs 615 nm).
Antibody studies of cells and tissues
that have Fc-receptors often are complicated by non-specific reactions,
which are greatly diminished if the Fc part of antibodies is removed
to produce F(ab')2 fragments. PROTOS IMMUNORESEARCH prepares
antibody F(ab')2 fragments both as unconjugated and conjugated
reagents for immunocytological and tissue investigations.
In addition, FITC conjugated Fab' fragments
of affinity purified antibodies are provided for studies where increased
tissue or cellular penetration are important. The Fab' (i.e.3.5S)
fragments are monovalent (non-crosslinking).
Depending upon the technology employed, the usual working
dilutions for fluorochrome conjugates are >1:50 and <1:300.
Indicator : antibody ratios are as follows:
FITC conjugates, Mol.F/P ratios-whole Abs :4-6
conjugates OD515/OD280 :0.5-0.8
Red conjugates OD596/OD280 :0.6-1.0
875A Cowan Road, Burlingame, CA 94010
Tel: 650 652-3450
Fax: 650 652-3451
Information> Technical Information on Fluorochrome Antibody